A team of authors, led by John P.A. Ioannidis, analyzed a randomly chosen set of 441 PubMed-indexed papers related to biomedicine which were published between 2000 and 2014. Among these 441 publications, 268 included empirical data. Of these 268, only one study provided a complete and full protocol. None of the selected 268 papers provided means to access full datasets; only one mentioned making complete raw data available upon request. The majority of studies did not contain any conflict of interest statement (69.2%) and only half of all papers included funding information (51.7%). Interestingly, only 1.5% papers with empirical data were replication studies. Altogether these results demonstrate the continued need for improving reproducibility and transparency practices in the biomedical literature. (PLOS Biology, DOI:10.1371/journal.pbio.1002333 January 4, 2016).