In this article, the authors introduce The Brazilian Reproducibility Initiative, a multi-center effort to estimate the reproducibility of Brazilian biomedical science. The initiative aims to repeat between 60 and 100 experiments in a systematic manner: the project will focus on a set of common laboratory methods, repeating each experiment in three different laboratories. The results, due in 2021, will allow to estimate the level of reproducibility of biomedical science in Brazil, and to investigate what the published literature can tell us about the reproducibility of research in a given area.